Japanese Journal of Veterinary Research;Volume 52, Number 4


Examination of the Lunatic fringe and Uncx4.1 expression by whole-mount in situ hybridization in the embryo of the CKH-Jsr(jumbled spine and ribs)mouse

OKANO, Shinya;ASANO, Atsushi;SASAKI, Nobuya;KON, Yasuhiro;WATANABE, Tomomasa;AGUI, Takashi

Permalink : http://hdl.handle.net/2115/873
JaLCDOI : 10.14943/jjvr.52.4.145
KEYWORDS : Jsr;Lfng;Uncx4.1


The CKH-Jsr (jumbled spine and ribs) mouse was found as a spontaneous mutant with malformation of vertebrae, that is, a short trunk and kinky tail.We examined Lunatic Fringe (Lfng) and Uncx4.1 expression in the presomitic mesoderm (PSM) and somites of Jsr-mutant (CKH-Jsr/+) embryos to elucidate pathogenesis of the Jsr mutation. Expression pattern of Lfng in the PSM of Jsr-mutant embryos was similar to that of the normal (C57BL/6) embryos. However, expression pattern of Uncx4.1 in the somites of Jsrmutant embryos was impaired to be irregular and mosaic, suggesting that the anterior-posterior (A-P) polarity is disordered in the Jsr mutant. These results indicate that the Jsr mutation disrupts the A-P polarity of somites during the somitogenesis without altering Lfng expression pattern in the PSM.