Japanese Journal of Veterinary Research;Volume 39, Number 2-4

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CHARACTERIZATION OF DNA FRAGMENT FROM CHLAMYDIA PSITTACI AVIAN STRAIN WHICH SHOWS HIGH HOMOLOGY WITH HYPB GENE OF CHLAMYDIA

SATO, Chiaki;KATUMATA, Atushi;TAKASHIMA, Ikuo;HASHIMOTO, Nobuo

Permalink : http://hdl.handle.net/2115/8940
JaLCDOI : 10.14943/jjvr.39.2-4.167
KEYWORDS : Chlamydia psittaci;heat-shock gene

Abstract

A study was performed to characterize DNA fragment No. 17 of C. psittaci strain P-1041 which encoded 42 KD β-galactosidase fusion protein with typespecific antigenecity. Sequence determination identified a partial open reading frame that spanned about 1,200b. p. nucleotides. Screening the literatures for the nucleotide and deduced amino acid sequences revealed extensive similarity between the DNA fragment of P-1041 and two chlamydial hypB genes. This DNA showed 91.5% homology with C. psittaci GPIC hypB gene in nucleotide sequence and 96.4% homology in deduced amino acid sequence. The hypB gene of C. trachomatis serovar A and the P-1041 DNA fragment showed 81.2% and 91.3% homology in nucleotide and amino acid sequences, respectively. Dot enzyme-linked immunosorbent assay, for the products of deleted DNA fragments defined the coding region for type-specific antigenic polypeptide. In addition, the P-1041 DNA fragment carried a sequence highly homologous (>49%) with other bacterial and plant genes called chaperonin which responds to various stress in cells. From these results, the P-1041 DNA fragment was found to be a part of hypB gene and to encode the region critical for type-specific antigenicity.

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