Japanese Journal of Veterinary Research;Volume 66 Number 4

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Genetic diversity and global relationships of Mycoplasma hyopneumoniae from slaughter age pigs in Chiang Mai and Lamphun Provinces, Thailand

Tadee, Pakpoom;Chanrittisen, Korarit;Thainoi, Natthaporn;Thongkamkoon, Patcharee;Patchanee, Prapas

Permalink : http://hdl.handle.net/2115/72018
JaLCDOI : 10.14943/jjvr.66.4.251
KEYWORDS : enzootic pneumonia;MLST;Mycoplasma hyopneumoniae;pig;Thailand

Abstract

Mycoplasma hyopneumoniae is a causative agent of enzootic pneumonia concerning for economic burdens in pig herds. Detection in genetic variable of the agent is an essential tool, which can be traced the route of disease spreading. Multilocus Sequence Typing (MLST) is accepted as one of the methods widely used. This study investigated genotypic characteristics of 16 M. hyopneumoniae strains recovered from consolidated lungs of slaughter age pigs in Chiang Mai and Lamphun provinces during 2016-2017 and 229 pig-originated strains previously submitted to global MLST database during 2001-2017, focusing on the locus numbers of three housekeeping genes, adk, rpoB and tpiA in MLST analysis to expand an understanding of the epidemiological knowledge for enzootic pneumonia. Of the 16 local strains, ten novel-first reported sequence types (ST99-ST108) from 14 strains were discovered, while the remaining 2 were identified in ST48. ST102 denoted for the majority, which accounted for four strains. Strains delivered at different collection times were not clones. However, some groups of strains collected at identical period belonging long distances were determined to be clones. At the global level, one hundred and eight STs were distributed in 15 countries. Almost all strains grouped in any STs were allied to the single country. The one exception is the strains grouped in ST48, which were derived from Thailand and Greece. In summary, MLST has the advantage of providing precise typing outcomes for epidemiology. The findings from this study provide advice regarding effective prevention and control of enzootic pneumonia in the study area.

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