Japanese Journal of Veterinary Research;Volume 66 Number 4

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Effect of astaxanthin addition to an individual culture system for in vitro maturation of bovine oocytes on accumulation of reactive oxygen species and mitochondrial activity

Erdenetogtokh, Purevdorj;Kanno, Chihiro;Sakaguchi, Kenichiro;Yanagawa, Yojiro;Katagiri, Seiji;Nagano, Masashi

Permalink : http://hdl.handle.net/2115/72027
JaLCDOI : 10.14943/jjvr.66.4.325
KEYWORDS : IVM;mitochondria;ROS

Abstract

We investigated the effects of astaxanthin (Ax) on the accumulation of reactive oxygen spices (ROS) and mitochondrial activity in bovine oocytes during individual maturational culture. Oocytes were cultured individually for 22 h in multi-well plates with or without 500 ng/ml Ax. After maturational culture, ROS accumulation of oocytes was lower in Ax-treated oocytes than in non-treated oocytes (P < 0.05). Mitochondrial activity was higher in Ax-treated oocytes than in non-treated oocytes (P < 0.05). The development of blastocysts in the Ax-treated oocytes tended to be higher than in non-treated oocytes (P = 0.06). These results indicate that Ax treatment improves the developmental competence of bovine oocytes, maybe due to the reduction of ROS accumulation and the improvement of mitochondrial activity.

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