Japanese Journal of Veterinary Research;Volume 67 Number 3

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Hydroxyl radical formation in lactated Ringer’s solution and BSS Plus® intraocular irrigating solution

Naganobu, Kiyokazu;Nakajima, Akira;Kaneko, Yasuyuki;Torisu, Shidow;Sekiguchi, Satoshi;Chuman, Hideki

Permalink : http://hdl.handle.net/2115/75486
JaLCDOI : 10.14943/jjvr.67.3.209
KEYWORDS : free radical;hydroxyl radical;phacoemulsification

Abstract

Free radicals generated during phacoemulsification, a method of cataract surgery, have been shown to damage corneal endothelial cells. We compared hydroxyl radical formation (HRF) in lactated Ringer’s solution (LRS) and BSS Plus® intraocular irrigating solution, as well as physiological saline solution (PSS) and Ringer’s solution (RS). Hydroxyl radicals were generated by a phacoemulsification machine or UV irradiation in the presence of hydrogen peroxide. We measured DMPO-hydroxyl radical adducts using ESR. HRF did not significantly differ between LRS and BSS Plus®, and tended to be smaller in LRS and BSS Plus® than in PSS or RS. Even if corneal damage after phacoemulsification in LRS is different from that in BSS Plus®, the influence of HRF on this difference may be small.

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