北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University;第70巻 第2号

FONT SIZE:  S M L

Intersubfamilial Hybridization of Two Danio and Six Related Cyprinid Fishes

Umezawa, Ryuhei;Urushibata, Hirotaro;Takahashi, Eisuke;Arai, Katsutoshi;Yamaha, Etsuro

Permalink : http://hdl.handle.net/2115/80066
JaLCDOI : 10.14943/bull.fish.70.2.133
KEYWORDS : Chimera;Distant hybrid;DNA content;Flow cytometry;Genome size;Polyploid

Abstract

Production of sterile individuals is the key technique for surrogate propagation in teleosts. Sterile hybrids may be the ideal surrogate host when they do not generate their own germ cells in their gonads. Here, we attempted hybridization experiments between zebrafish (Danio rerio) and six closely related species (Danio albolineatus, Aphycypris chinensis, Hemigrammocypris rasborella, Opsariichthys platypus, Nipponocypris temminckii, N. sieboldii) and one remotely related species (Tanichthys albonubes). Intersubfamilial hybridizations in the family Cyprinidae resulted in the occurrence of inviable abnormal larvae with the two parental genomes, except for the T. albonubes x A. chinensis hybridization, in which normal larvae survived. Allotetraploidy and spontaneous gynogenetic diploidy were infrequently detected in T. albonubes x A. chinensis and D. albolineatus x A. chinensis, respectively

FULL TEXT:PDF