Japanese Journal of Veterinary Research;Volume 69 Number 1


Molecular characterization of a Newcastle disease virus isolate from a diseased chicken in the Philippines in 2017

Tran, Giang Thi Huong;Mananggit, Milagros Reyes;Abao, Lary Nel Bilbao;Dong, Hieu Van;Takeda, Yohei;Ogawa, Haruko;Imai, Kunitoshi

Permalink : http://hdl.handle.net/2115/80623
JaLCDOI : 10.14943/jjvr.69.1.73
KEYWORDS : Genotype VII;Newcastle disease virus;The Philippines


Newcastle disease (ND) is one of the most serious diseases affecting numerous avian species worldwide. However, data on the genetic characterization of ND virus (NDV) in the Philippines are limited. Herein, the complete sequences of the fusion (F) and hemagglutinin-neuraminidase (HN) genes of a NDV strain isolated in Central Luzon in 2017 were genetically analyzed. The results revealed that the field isolate belonged to subgenotype VII.2 and the genome encoded the amino acid sequence of the F0 protein proteolytic cleavage site motif (112R-R-Q-K-R-F117), indicating that it was a virulent strain. The current NDV strain was genetically related to Indonesian and Israeli NDV strains. Some amino acid substitutions were found in the transmembrane domain and neutralizing epitopes of the HN protein.

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