Japanese Journal of Veterinary Research;Volume 16, Number 1

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EFFECT OF SODIUM DESOXYCHOLATE ON INFECTIOUS CANINE HEPATITIS VIRUS, AND AN ADDITIONAL DESCRIPTION OF A SMALL ASSOCIATED VIRUS

SUGIMURA, Takaaki;YANAGAWA, Ryo

Permalink : http://hdl.handle.net/2115/1893
JaLCDOI : 10.14943/jjvr.16.1.1

Abstract

The soluble antigen of ICH virus was fractionated by DEAE cellulose column chromatography and two antigens, CF and HA antigens, were separated. CF antigen was eluted at 0.05M NaCl, HA antigen was eluted at 0.2 M NaCl, both in 0.01 M phosphate buffer solution. The CF antigenicity shown by the HA antigen fraction differed from that of the CF antigen fraction. The ICH virus particle could be destroyed by treatment with 0.5% sodium desoxycholate (SDC), consequently its infectivity was lost, CF titer was increased and HA titer was decreased. When SDC was removed by dialysis, the changed CF and HA titers returned to the original levels. The destruction of the virus particle by SDC was shown electron microscopically. Also the aggregation of free capsomeres was morphologically observed after SDC was removed. Discussion was carried out on the change due to SDC, the recovery due to removal of SDC, of the CF and HA antigen titers. The results obtained agreed in that CF and HA antigens are the structural components of ICH virus. Existence of ICH-associated or satellite virus was proved electron microscopically.

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