Japanese Journal of Veterinary Research;Volume 52, Number 2

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Effects of oxygen tension in the gas stmosphere during in vitro maturation, in vitro fertilization and in vitro culture on the efficiency of in vitro production of mouse embryos

Adam, Ahmed Adbel Gadir;Takahashi, Yoshiyuki;Katagiri, Seiji;Nagano, Masashi

Permalink : http://hdl.handle.net/2115/10512
KEYWORDS : development;fertization;maturation;mouse oocyte;oxygen tension

Abstract

Effects of oxygen (O₂) tension in the gas atmosphere during in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) on the efficiency of in vitro production of mouse embryos were examined. Mouse oocytes recoverd from large antral follicles were subjected to IVM in Waymouth medium for 15, 16and 17 hr under 5 or 20% O₂ and then subjected to IVF and IVC under 5 or 20% O₂ tension. Lawering the O₂ tension on the gas atmosphere for IVM from 20 to 5% improved the cleavage rate after IVF when the oocytes were subjected to IVM for 15 hr; however, no improvement in the cleavage rate was observed when the culture period for IVM was extended to 16 and 17 hr. Lowering the O₂ tension to 5% for IVM and IVC improved the development of the cleaved oocytes to the blastocyst stage, regardless of the culture period for IVM. However, the O₂ tension for IVF had no remarkable effect on the subsequent embryonic development. These results demonstrate that 5% O₂ is superior to 20% O2 for IVM and IVC, and suggest that 20% O₂ for IVM may delay oocyte maturation and/or the acquisition of fertilizability and impair the developmental competence of oocytes.

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