Characterization of inner capsid σA protein as a virulence factor of the pteropine orthoreovirus

Harima, Hayato; Sasaki, Michihito; Ariizumi, Takuma; Kawaguchi, Nijiho; Kobayashi, Hiroko; Intaruck, Kittiya; Kobayashi, Takeshi; Kawagishi, Takahiro; Kanai, Yuta; Nao, Naganori; Qiu, Yongjin; Kajihara, Masahiro; Orba, Yasuko; Ito, Naoto; Ishihara, Kanako; Saijo, Masayuki; Hall, William W.; Hang’ombe, Bernard M.; Sawa, Hirofumi

Characterization of inner capsid σA protein as a virulence factor of the pteropine orthoreovirus

Harima, Hayato; Sasaki, Michihito; Ariizumi, Takuma et al.

PLOS Pathogens, 2026, 22(5), e1014252

Permalink : https://hdl.handle.net/2115/100448

このアイテムのアクセス数:22件（2026-10-17 12:31 集計）

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https://doi.org/10.1371/journal.ppat.1014252			11

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アクセス権	open access
URI	https://hdl.handle.net/2115/100448
タイトル	Characterization of inner capsid σA protein as a virulence factor of the pteropine orthoreovirus
著者	Harima, Hayato ; Sasaki, Michihito ; Ariizumi, Takuma ; Kawaguchi, Nijiho ; Kobayashi, Hiroko ; Intaruck, Kittiya ; Kobayashi, Takeshi ; Kawagishi, Takahiro ; Kanai, Yuta ; Nao, Naganori ; Qiu, Yongjin ; Kajihara, Masahiro ; Orba, Yasuko ; Ito, Naoto ; Ishihara, Kanako ; Saijo, Masayuki ; Hall, William W. ; Hang’ombe, Bernard M. ; Sawa, Hirofumi
著者名 Harima, Hayato
著者名 Sasaki, Michihito
著者名 Ariizumi, Takuma
著者名 Kawaguchi, Nijiho
著者名 Kobayashi, Hiroko
著者名 Intaruck, Kittiya
著者名 Kobayashi, Takeshi
著者名 Kawagishi, Takahiro
著者名 Kanai, Yuta
著者名 Nao, Naganori
著者名 Qiu, Yongjin
著者名 Kajihara, Masahiro
著者名 Orba, Yasuko
著者名 Ito, Naoto
著者名 Ishihara, Kanako
著者名 Saijo, Masayuki
著者名 Hall, William W.
著者名 Hang’ombe, Bernard M.
ORCID 0000-0003-2569-2755 著者名 Sawa, Hirofumi
言語	英語
発行日	2026-05-26
出版者	Public Library of Science (PLoS)
収録物名	PLOS Pathogens
巻(号)	22(5)
ページ	e1014252
抄録	Pteropine orthoreovirus (PRV) is an emerging zoonotic virus that causes pneumonia in humans. We previously isolated the PRV strain Nachunsulwe-57 (N57) from a Zambian fruit bat and demonstrated its low virulence in laboratory mice. Here, we have attempted to identify factors responsible for differences in the virulence between strain N57 and the human-derived clinical strain Miyazaki-Bali/2007 (MB). Characterization of the virulence of recombinant monoreassortant PRVs derived from highly virulent MB and low virulent N57 strains in mice revealed that compared with wild-type (WT) MB, MB-based monoreassortants carrying the L1, S1, or S2 segment from N57 exhibited attenuated virulence. Among these, the monoreassortants carrying the S1 or S2 segment exhibited reduced viral loads and reduced cytokine gene expression levels in the lungs. Genetic mapping of virulence determinants using the reciprocal monoreassortant viruses with increased virulence demonstrated that N57-based monoreassortants carrying the S1 or S2 segment of MB exhibited enhanced virulence, resulting in lower survival rate compared with WT N57. Unlike the S1 segment, the functions of the S2 segment in pathogenesis are unclear. Thus, we further investigated the functional region of the inner-capsid σA protein encoded by the S2 segment. Notably, Ser-46 of σA was identified as a key amino acid determinant of PRV virulence and is present in strains derived from humans, monkeys, and bat flies, but not those identified from bats (their natural host). Collectively, these findings demonstrate that PRV σA is one factor that regulates virulence, and that σA Ser-46 may be related to potential interspecies transmission events from bats.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
EISSN	1553-7374
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.ppat.1014252