Hypoxia enhances the expression of autocrine motility factor and the motility of human pancreatic cancer cells

Niizeki, Hiroto; Kobayashi, Masanobu; Horiuchi, Iori; Akakura, Nobuaki; Chen, Jian; Wang, Jingxin; Hamada, Jun-ichi; Seth, Prem; Katoh, Hiroyuki; Watanabe, Hideomi; Raz, Avraham; Hosokawa, Masuo

Hypoxia enhances the expression of autocrine motility factor and the motility of human pancreatic cancer cells

Niizeki, Hiroto; Kobayashi, Masanobu; Horiuchi, Iori et al.

British Journal of Cancer, 2002, 86(12), 1914-1919

Permalink : https://hdl.handle.net/2115/11317

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アクセス権	open access
URI	https://hdl.handle.net/2115/11317
タイトル	Hypoxia enhances the expression of autocrine motility factor and the motility of human pancreatic cancer cells
著者	Niizeki, Hiroto ; Kobayashi, Masanobu ; Horiuchi, Iori ; Akakura, Nobuaki ; Chen, Jian ; Wang, Jingxin ; Hamada, Jun-ichi ; Seth, Prem ; Katoh, Hiroyuki ; Watanabe, Hideomi ; Raz, Avraham ; Hosokawa, Masuo
著者名 Niizeki, Hiroto
著者名 Kobayashi, Masanobu
著者名 Horiuchi, Iori
著者名 Akakura, Nobuaki
著者名 Chen, Jian
著者名 Wang, Jingxin
著者名 Hamada, Jun-ichi
著者名 Seth, Prem
著者名 Katoh, Hiroyuki
著者名 Watanabe, Hideomi
著者名 Raz, Avraham
著者名 Hosokawa, Masuo
言語	英語
キーワード	hypoxia
	AMF
	motility
	HIF-1
	metastasis
発行日	2002-06-17
出版者	Nature Publishing Group
収録物名	British Journal of Cancer
巻(号)	86(12)
ページ	1914-1919
抄録	The incidence of distant metastases is higher in the tumors with low oxygen pressure than in those with high oxygen pressure. It is well known that hypoxia induces the transcription of various genes involved in angiogenesis and anaerobic metabolism necessary for the growth of tumor cells in vivo, suggesting that hypoxia may also induce the transcription of metastasis-associated genes. We sought to identify the metastasis-associated genes differentially expressed in tumor cells under hypoxic conditions with the use of a DNA microarray system. We found that hypoxia enhanced the expression of autocrine motility factor (AMF) mRNA in various cancer cells and also enhanced the random motility of pancreatic cancer cells. AMF inhibitors abrogated the increase of motility under hypoxic conditions. In order to explore the roles of hypoxia-inducible factor-1α (HIF-1α), we established HIF-1α-transfectants and dominant negative HIF-1α-transfectants. Transfection with HIF-1α and dominant-negative HIF-1α enhanced and suppressed the expression of AMF/PHI/NL mRNA and the random motility, respectively. These results suggest that hypoxia may promote the metastatic potential of cancer cells through the enhanced AMF/PHI/NL mRNA expression and that the disruption of the HIF-1 pathway may be an effective treatment for metastasis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0007-0920
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1038/sj.bjc.6600331