Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells

TAKAHASHI, Eriko; INANAMI, Osamu; ASANUMA, Taketoshi et al.

Journal of Radiation Research, 2006, 47(1), 19-25

Permalink : https://hdl.handle.net/2115/11387

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アクセス権
open access
URI
https://hdl.handle.net/2115/11387
タイトル
Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells
著者
TAKAHASHI, Eriko ; INANAMI, Osamu ; ASANUMA, Taketoshi ; KUWABARA, Mikinori
著者名
TAKAHASHI, Eriko
e-Rad_Researcher
10193559
著者名
INANAMI, Osamu
著者名
ASANUMA, Taketoshi
著者名
KUWABARA, Mikinori
言語
英語
キーワード
Ceramide
Fumonisin B1
MOLT-4
Sphingomyelinase
X irradiation
発行日
2006-03-30
出版者
日本放射線影響学会
収録物名
Journal of Radiation Research
巻(号)
47(1)
ページ
19-25
抄録
In the present study, using inhibitors of ceramide synthase (fumonisin B1), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B1 and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0449-3060
EISSN
1349-9157
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1269/jrr.47.19