Changes in S1P(1) and SIP(2) expression during embryonal development and primitive endoderm differentiation of F9 cells

Hiraga, Yuki; Kihara, Akio; Sano, Takamitsu; Igarashi, Yasuyuki

Changes in S1P(1) and SIP(2) expression during embryonal development and primitive endoderm differentiation of F9 cells

Hiraga, Yuki; Kihara, Akio; Sano, Takamitsu et al.

Biochemical and biophysical research communications, 2006, 344(3), 852-858

Permalink : https://hdl.handle.net/2115/11391

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アクセス権	open access
URI	https://hdl.handle.net/2115/11391
タイトル	Changes in S1P(1) and SIP(2) expression during embryonal development and primitive endoderm differentiation of F9 cells
著者	Hiraga, Yuki ; Kihara, Akio ; Sano, Takamitsu ; Igarashi, Yasuyuki
著者名 Hiraga, Yuki
e-Rad_Researcher 50333620 著者名 Kihara, Akio
e-Rad_Researcher 10399967 著者名 Sano, Takamitsu
e-Rad_Researcher 70091965 著者名 Igarashi, Yasuyuki
言語	英語
キーワード	Sphingolipid
	Sphingosine 1-phosphate
	S1P receptor
	S1P(1)
	embryonal development
	differentiation
	endoderm
	F9 cells
発行日	2006-06-09
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
収録物名	Biochemical and biophysical research communications
巻(号)	344(3)
ページ	852-858
抄録	Sphingosine 1-phosphate (S1P) is a ligand for S1P family receptors (S1P1–S1P5). Of these receptors, S1P1, S1P2, and S1P3 are ubiquitously expressed in adult mice, while S1P4 and S1P5 are tissue specific. However, little is known of their expression during embryonal development. We performed Northern blot analyses in mouse embryonal tissue and found that such expression is developmentally regulated. We also examined the expression of these receptors during primitive endoderm (PrE) differentiation of mouse F9 embryonal carcinoma (EC) cells, a well-known in vitro endoderm differentiation system. S1P2 mRNA was abundantly expressed in F9 EC cells, but little S1P1 and no S1P3, S1P4, or S1P5 mRNA was detectable. However, S1P1 mRNA expression was induced during EC-to-PrE differentiation. Studies using small interference RNA of S1P1 indicated that increased S1P1 expression is required for PrE differentiation. Thus, S1P1 may play an important function in PrE differentiation that is not substituted for by S1P2.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
関連情報	PMID 16631609
関連情報	URI http://www.sciencedirect.com/science/journal/0006291X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2006.04.002