open access

https://hdl.handle.net/2115/13481

Selective Foam Separation of Binary Protein Solution

Selective Foam Separation of Binary Protein Solution by SDS Complexation Method.

英語

2002-09-15

Elsevier

Journal of Colloid and Interface Science

253(2)

402-408

A fundamental study about the selective foam separation of protein mixture was carried out. A solution containing two proteins, Ovalbumin (OA) and Lysozyme (LZ), and an anionic surfactant, Sodium Dodecyle Sulfate (SDS), was adjusted to pH 6.0 which refered to an intermediate state between the isoelectric points of the proteins. The solution was processed by continuous foam separation. The results showed that a proper addition of SDS greatly improved the selective recovery of LZ to OA. The experimental data were well explained by a simple model that most of cationic protein molecules (LZ) are associated with SDS and the adsorption of all the species including LZ-SDS complexes are subjected to Langmuir adsorption isotherm. The results also showed that one of the Langmuir parameters, which means a kind of lyophillic property of adsorbed material, of LZ-SDS complexes was extremely large as compared with that of primary protein.

学術雑誌論文

Accepted Manuscript

Copyright © 2002 Elsevier

0021-9797