Specificity of hybridization between DNA sequences based on free energy

Tanaka, F.; Kameda, A.; Yamamoto, M. et al.

DNA COMPUTING, 2006, 3892, 371-379

Permalink : https://hdl.handle.net/2115/14471

このアイテムのアクセス数:6,508件（2026-02-09 15:17 集計）

ファイル	フォーマット	サイズ	閲覧回数	説明
Fumiaki	pdf	257 KB	1,538

アクセス権	open access
URI	https://hdl.handle.net/2115/14471
タイトル	Specificity of hybridization between DNA sequences based on free energy
著者	Tanaka, F. ; Kameda, A. ; Yamamoto, M. ; Ohuchi, A.
著者名 Tanaka, F. 田中, 文昭
著者名 Kameda, A.
e-Rad_Researcher 40292057 著者名 Yamamoto, M.
著者名 Ohuchi, A.
言語	英語
発行日	2006
出版者	Springer
収録物名	DNA COMPUTING
巻(号)	3892
ページ	371-379
抄録	We investigated the specificity of hybridization based on a minimum free energy (Delta G(min)) through gel electrophoresis analysis. The analysis, using 94 pairs of sequences with length 20, showed that sequences that hybridize each other can be separated using the constraint Delta G(min) < -14.0, but cannot be separated using the number of base pairs (BP) in the range from 9 to 18. This demonstrates that the Delta G(min) is superior to the BP in terms of the capability to separate specific from non-specific sequences. Furthermore, the comparison between sequence design based on Delta G(min) and that based on the BP, done through a computer simulation, showed that the former outperformed the latter in terms of the number of sequences designed successfully as well as the ratio of successfully designed sequences to the total number of sequences checked.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0302-9743
関連情報	URI http://www.springerlink.com