Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families

Nomura, Kazuharu; Morishima, Kagayaki; Tanaka, Hideki; Unuma, Tatsuya; Okuzawa, Koichi; Ohta, hiromi; Arai, Katsutoshi

Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families

Nomura, Kazuharu; Morishima, Kagayaki; Tanaka, Hideki et al.

Aquaculture, 2006, 257(1-4), 53-67

Permalink : https://hdl.handle.net/2115/14487

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アクセス権	open access
URI	https://hdl.handle.net/2115/14487
タイトル	Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families
著者	Nomura, Kazuharu ; Morishima, Kagayaki ; Tanaka, Hideki ; Unuma, Tatsuya ; Okuzawa, Koichi ; Ohta, hiromi ; Arai, Katsutoshi
著者名 Nomura, Kazuharu 野村, 和晴
著者名 Morishima, Kagayaki 森島, 輝
著者名 Tanaka, Hideki
著者名 Unuma, Tatsuya
著者名 Okuzawa, Koichi
著者名 Ohta, hiromi
e-Rad_Researcher 00137902 著者名 Arai, Katsutoshi
言語	英語
キーワード	Anguilla japonica
	gene-centromere mapping
	half-tetrad analysis
	interference
	microsatellite
	triploid
発行日	2006-06-30
出版者	Elsevier
収録物名	Aquaculture
巻(号)	257(1-4)
ページ	53-67
抄録	Genetic improvement of the Japanese eel (Anguilla japonica) can be achieved by artificially controlling its life cycle using recent advances in reproductive biology. In this study, we developed 43 microsatellite loci to confirm Mendelian inheritance at 10 of them as well at 16 previously reported in two full-sib families produced by artificial insemination. In order to establish a base for aquaculture genetics of this species in the near future, these microsatellite loci were mapped in relation to the centromere by half-tetrad analysis using four artificially induced triploid families. The second division segregation frequency (y) of the microsatellite loci ranged from 0.008 to 0.968 (mean ± SD = 0.645 ± 0.298). These results suggest the presence of strong chiasma interference in the eel. Significant differences were observed for the map distances of microsatellite loci between the two isolation procedures. Microsatellites isolated using the enrichment procedure were mapped to various sites starting from the centromere to the telomere, whereas those from the conventional size-selected library showed a tendency to be distributed in the telomeric region.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0044-8486
関連情報	URI http://www.sciencedirect.com/science/journal/00448486
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.aquaculture.2006.03.011