Ammonia Channel Couples Glutaminase with Transamidase Reactions in GatCAB

Nakamura, Akiyoshi; Yao, Min; Chimnaronk, Sarin et al.

Science, 2006, 312(5782), 1954-1958

Permalink : https://hdl.handle.net/2115/14490

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アクセス権
open access
URI
https://hdl.handle.net/2115/14490
タイトル
Ammonia Channel Couples Glutaminase with Transamidase Reactions in GatCAB
著者
Nakamura, Akiyoshi ; Yao, Min ; Chimnaronk, Sarin ; Sakai, Naoki ; Tanaka, Isao
著者名
Nakamura, Akiyoshi
中村, 彰良
e-Rad_Researcher
40311518
著者名
Yao, Min
著者名
Chimnaronk, Sarin
著者名
Sakai, Naoki
e-Rad_Researcher
70093052
著者名
Tanaka, Isao
田中, 勲
言語
英語
発行日
2006-06-30
出版者
American Association for the Advancement of Science
収録物名
Science
巻(号)
312(5782)
ページ
1954-1958
抄録
The formation of glutaminyl. transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 angstrom in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gin-tRNA(Gln).
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0036-8075
EISSN
1095-9203
関連情報
PMID
16809541
URI
http://www.sciencemag.org/cgi/content/full/312/5782/1954
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1126/science.1127156