From phasing to structure refinement in-house : Cr/Cu dual-wavelength system and a loopless free crystal-mounting method

Watanabe, Nobuhisa

Acta Crystallographica Section D: Biological Crystallography, 2006, 62(8), 891-896

Permalink : https://hdl.handle.net/2115/14526

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アクセス権
open access
URI
https://hdl.handle.net/2115/14526
タイトル
From phasing to structure refinement in-house : Cr/Cu dual-wavelength system and a loopless free crystal-mounting method
著者
著者名
Watanabe, Nobuhisa
言語
英語
キーワード
Cr radiation
SAD method
free crystal mounting method
発行日
2006-08
出版者
International Union of Crystallography
収録物名
Acta Crystallographica Section D: Biological Crystallography
巻(号)
62(8)
ページ
891-896
抄録
The practical applicability of in-house structure determination using Cr Kα X-rays (2.29 Å) and a loopless free crystal mounting method was examined using five novel proteins. Proteins from 9.6 kDa to 84 kDa have been solved using this method without any derivatization. In all cases, more than 90% of all the structures were constructed automatically with side chains by use of the Cr SAD method. The free crystal mounting technique increases the accuracy of the anomalous differences between the Bijvoet mates, and makes the in-house singlewavelength SAD method with a Cr Kα X-ray source a very useful tool for high-throughput structure determination. In addition, a Cr/Cu dual wavelength system makes it possible to perform structure analysis from phasing to refinement of the structure in-house.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
Copyright © International Union of Crystallography
PISSN
0907-4449
関連情報
PMID
16855305
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1107/S0907444906010432