Two-component bacterial multidrug transporter, EbrAB: Mutations making each component solely functional

Kikukawa, Takashi; Nara, Toshifumi; Araiso, Tsunehisa; Miyauchi, Seiji; Kamo, Naoki

Two-component bacterial multidrug transporter, EbrAB: Mutations making each component solely functional

Kikukawa, Takashi; Nara, Toshifumi; Araiso, Tsunehisa et al.

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2006, 1758(5), 673-679

Permalink : https://hdl.handle.net/2115/14584

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アクセス権	open access
URI	https://hdl.handle.net/2115/14584
タイトル	Two-component bacterial multidrug transporter, EbrAB: Mutations making each component solely functional
著者	Kikukawa, Takashi ; Nara, Toshifumi ; Araiso, Tsunehisa ; Miyauchi, Seiji ; Kamo, Naoki
e-Rad_Researcher 20281842 著者名 Kikukawa, Takashi
著者名 Nara, Toshifumi
著者名 Araiso, Tsunehisa
著者名 Miyauchi, Seiji
著者名 Kamo, Naoki
言語	英語
キーワード	EbrAB
	EmrE
	Smr
	Ion-coupled transporter
	Multidrug resistance
発行日	2006-05
出版者	Elsevier B.V.
収録物名	Biochimica et Biophysica Acta (BBA) - Biomembranes
巻(号)	1758(5)
ページ	673-679
抄録	EbrAB in Bacillus subtilis belongs to a novel small multidrug resistance (SMR) family of multidrug efflux pumps. EmrE in Escherichia coli, a representative of SMR, functions as a homo-oligomer in the membrane. On the other hand, EbrAB requires a hetero-oligomeric configuration consisting of two polypeptides, EbrA and EbrB. Although both polypeptides have a high sequence similarity, expression of either single polypeptide does not confer the multidrug-resistance. We performed mutation studies on EbrA and B to determine why EbrAB requires the hetero-oligomerization. Mutants of EbrA and B lacking both the hydrophilic loops and the C-terminus regions conferred the multidrug-resistance solely by each protein. This suggests that the hydrophilic loops and the C-terminus regions constrain them to their respective conformations upon the formation of the functional hetero-oligomer.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0005-2736
関連情報	PMID 16750162
関連情報	URI http://www.sciencedirect.com/science/journal/00052736
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbamem.2006.04.004