A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1

Orikasa, Yoshitake; Nishida, Takanori; Hase, Akira; Watanabe, Kazuo; Morita, Naoki; Okuyama, Hidetoshi

A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1

Orikasa, Yoshitake; Nishida, Takanori; Hase, Akira et al.

FEBS Letters, 2006, 580(18), 4423-4429

Permalink : https://hdl.handle.net/2115/14652

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/14652
タイトル	A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1
著者	Orikasa, Yoshitake ; Nishida, Takanori ; Hase, Akira ; Watanabe, Kazuo ; Morita, Naoki ; Okuyama, Hidetoshi
著者名 Orikasa, Yoshitake
著者名 Nishida, Takanori
著者名 Hase, Akira
著者名 Watanabe, Kazuo
著者名 Morita, Naoki
e-Rad_Researcher 90125295 著者名 Okuyama, Hidetoshi
言語	英語
キーワード	Docosahexaenoic acid
	Eicosapentaenoic acid
	n−3 Polyunsaturated fatty acid
	Phosphopantetheinyl transferase
	pfaE gene
	Moritella marina strain MP-1
発行日	2006-08-07
出版者	Elsevier B.V.
収録物名	FEBS Letters
巻(号)	580(18)
ページ	4423-4429
抄録	A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA–D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n−3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0014-5793
関連情報	PMID 16859689
関連情報	URI http://www.sciencedirect.com/science/journal/00145793
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.febslet.2006.07.008