Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens

Naitoh, Y.; Ishibashi, Y.; Abe, S. et al.

Molecular Ecology Notes, 2002, 2(4), 434-436

Permalink : https://hdl.handle.net/2115/1471

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アクセス権	open access
URI	https://hdl.handle.net/2115/1471
タイトル	Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens
著者	Naitoh, Y. ; Ishibashi, Y. ; Abe, S. ; Ohdachi, S. D.
著者名 Naitoh, Y.
著者名 Ishibashi, Y.
e-Rad_Researcher 80125278 著者名 Abe, S.
e-Rad_Researcher 60292041 著者名 Ohdachi, S. D. 大舘, 智志
言語	英語
キーワード	cross-species amplification
	shrew
	simple sequence repeat
	Sorex
	Soricidae
	microsatellites
発行日	2002
出版者	Blackwell Publishing
収録物名	Molecular Ecology Notes
巻(号)	2(4)
ページ	434-436
抄録	We isolated six microsatellite markers from the partial genomic libraries of two Sorex shrews, S. unguiculatus and S. caecutiens, and examined their allelic variation. All loci showed high allelic variation ranging from 15 to 19 alleles and all but one locus conformed to Hardy-Weinberg expectations in the species where the loci were isolated. Cross-species amplifications showed that all primers derived from S. unguiculatus were useful for S. caecutiens, while among primer sets derived from S. caecutiens, only one was useful for S. unguiculatus. Accordingly, at least five microsatellite markers were useful in S. caecutiens and three in S. unguiculatus.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Copyright 2002 Blackwell Publishing
PISSN	1471-8278