Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) Gene of Arabidopsis Causes Weak rcd1 Phenotype with Compromised Oxidative-Stress Responses

Fujibe, Takahiro; Saji, Hikaru; Watahiki, Masaaki K.; Yamamoto, Kotaro T.

Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) Gene of Arabidopsis Causes Weak rcd1 Phenotype with Compromised Oxidative-Stress Responses

Fujibe, Takahiro; Saji, Hikaru; Watahiki, Masaaki K. et al.

Bioscience Biotechnology and Biochemistry, 2006, 70(8), 1827-1831

Permalink : https://hdl.handle.net/2115/14727

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アクセス権	open access
URI	https://hdl.handle.net/2115/14727
タイトル	Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) Gene of Arabidopsis Causes Weak rcd1 Phenotype with Compromised Oxidative-Stress Responses
著者	Fujibe, Takahiro ; Saji, Hikaru ; Watahiki, Masaaki K. ; Yamamoto, Kotaro T.
著者名 Fujibe, Takahiro
著者名 Saji, Hikaru
e-Rad_Researcher 70396282 著者名 Watahiki, Masaaki K.
e-Rad_Researcher 80142008 著者名 Yamamoto, Kotaro T.
言語	英語
キーワード	Arabidopsis thaliana
	methyl viologen (paraquat)
	ozone
	RCD1
	subnuclear localization
発行日	2006-08
出版者	Japan Society for Bioscience, Biotechnology, and Agrochemistry
収録物名	Bioscience Biotechnology and Biochemistry
巻(号)	70(8)
ページ	1827-1831
抄録	rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0916-8451
EISSN	1347-6947
関連情報	PMID 16926493
関連情報	URI http://www.jstage.jst.go.jp/
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1271/bbb.50673