open access

https://hdl.handle.net/2115/14776

Biofilm formation by a Bacillus subtilis strain that produces γ-polyglutamate

英語

2006

Society for General Microbiology

Microbiology

152(9)

2801-2807

The extracellular matrix produced by Bacillus subtilis B-1, an environmental strain that forms robust floating biofilms, was purified, and determined to be composed predominantly of -polyglutamate (-PGA), with a molecular mass of over 1000 kDa. Both biofilm formation and -PGA production by B. subtilis B-1 increased with increasing Mn2+ or glycerol concentration. -PGA was produced in a growth-associated manner in standing culture, and floating biofilms were formed. However, -PGA was produced in a non-growth-associated manner in shaking culture conditions. When B. subtilis B-1 was grown in a microaerated culture system, floating biofilm formation and -PGA production were significantly retarded, suggesting that oxygen depletion is involved in the initial steps of floating biofilm formation in standing culture. Proteomic analysis of membrane proteins demonstrated that flagellin, oligopeptide permease and Vpr protease precursor were the major proteins produced by cells in a floating biofilm and a colony.

学術雑誌論文

Version of Record

1350-0872

1465-2080