open access

https://hdl.handle.net/2115/14899

Rhythmic contraction and intracellular Ca2　+　 oscillatory rhythm in spontaneously beating cultured cardiac myocytes

英語

fluctuation

2005-10

Taylor & Francis

Biological Rhythm Research

36(4)

315-324

Cultured cardiac myocytes from neonatal rats show spontaneous and rhythmic contractions. The intracellular concentration of free Ca2 +  also changes rhythmically, associated with the rhythmic contraction of myocytes (Ca2 +  oscillation). This study aims to elucidate whether spontaneous rhythmic contraction affects the dynamics of intracellular Ca2 +  oscillation in cultured cardiac myocytes. In cultures at four days in vitro (4 DIV), spontaneous Ca2 +  oscillation was synchronized among myocytes. Treatment of cultures with an uncoupler of E – C coupling resulted in a cessation of the spontaneous contraction of cardiac myocytes, but did not abolish the Ca2 +  oscillation. The intercellular synchronization of intracellular Ca2 +  oscillation persisted, and both the intervals and the fluctuation of the oscillation tended to increase after the termination of rhythmic contraction. The present study demonstrated that mechanical factors associated with rhythmic contraction did not affect the intercellular synchronization of intracellular Ca2 +  oscillation, but possibly contributed to the stability of the oscillatory rhythm.

学術雑誌論文

Accepted Manuscript

0929-1016