A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.

Nagai, Nobuhiro; Yunoki, Shunji; Satoh, Yasuharu et al.

Journal of Bioscience and Bioengineering, 2004, 98(6), 493-496

Permalink : https://hdl.handle.net/2115/15816

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アクセス権
open access
URI
https://hdl.handle.net/2115/15816
タイトル
A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.
著者
Nagai, Nobuhiro ; Yunoki, Shunji ; Satoh, Yasuharu ; Tajima, Kenji ; Munekata, Masanobu
著者名
Nagai, Nobuhiro
著者名
Yunoki, Shunji
e-Rad_Researcher
30360928
著者名
Satoh, Yasuharu
e-Rad_Researcher
00271643
著者名
Tajima, Kenji
著者名
Munekata, Masanobu
言語
英語
キーワード
cell sheet
salmon atelocollagen
fibrillar gel
collagenase digestion
発行日
2004
出版者
Society for Bioscience and Bioengineering, Japan
収録物名
Journal of Bioscience and Bioengineering
巻(号)
98(6)
ページ
493-496
抄録
We prepared a cell sheet by using collagenase treatment to digest salmon atelocollagen fibrillar gel (SAC gel) on which human periodontal ligament (HPDL) cells had been cultured. The SAC gel was found to be digested completely within 2 h at a concentration of 50 U of collagenase per mg of collagen. The SAC gel on which HPDL cells were cultured for 10 d was treated with collagenase, resulting in the formation of a detached and shrunken cell sheet. Immunostaining results showed that the cytoskeleton and fibronectin matrix level of the cell sheet were maintained after collagenase treatment. In addition, collagenase treatment had almost no effect on the activities of HPDL cells.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
1389-1723
EISSN
1347-4421
関連情報
PMID
16233743
URI
http://www.sbj.or.jp/
URI
http://www.sciencedirect.com/science/journal/13891723
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/S1389-1723(05)00319-1