Application of cross-linked salmon atelocollagen to the scaffold of human periodontal ligament cells.

Nagai, Nobuhiro; Yunoki, Shunji; Suzuki, Takeshi; Sakata, Maiko; Tajima, Kenji; Munekata, Masanobu

Application of cross-linked salmon atelocollagen to the scaffold of human periodontal ligament cells.

Nagai, Nobuhiro; Yunoki, Shunji; Suzuki, Takeshi et al.

Journal of Bioscience and Bioengineering, 2004, 97(6), 389-394

Permalink : https://hdl.handle.net/2115/15817

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アクセス権	open access
URI	https://hdl.handle.net/2115/15817
タイトル	Application of cross-linked salmon atelocollagen to the scaffold of human periodontal ligament cells.
著者	Nagai, Nobuhiro ; Yunoki, Shunji ; Suzuki, Takeshi ; Sakata, Maiko ; Tajima, Kenji ; Munekata, Masanobu
著者名 Nagai, Nobuhiro
著者名 Yunoki, Shunji
著者名 Suzuki, Takeshi
著者名 Sakata, Maiko
e-Rad_Researcher 00271643 著者名 Tajima, Kenji
著者名 Munekata, Masanobu
言語	英語
キーワード	salmon atelocollagen
	scaffold
	cross-linking
	carbodiimide
	periodontal ligament cells
	denatured collagen
発行日	2004
出版者	Society for Bioscience and Bioengineering, Japan
収録物名	Journal of Bioscience and Bioengineering
巻(号)	97(6)
ページ	389-394
抄録	The purpose of this study was to investigate the application of salmon atelocollagen (SAC) to a scaffold. SAC has a low denaturation temperature and needs to be cross-linked before being used as a scaffold. In the present study, SAC was cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) or dehydrothermal treatment (DHT). The material properties (degree of cross-linking and solubility in phosphate-buffered saline) of the SAC scaffolds cross-linked by EDC (EDC-SAC) and DHT (DHT-SAC) were evaluated. It was found that EDC-SAC had a high degree of cross-linking and high stability compared with DHT-SAC. Human periodontal ligament (HPDL) cells were cultured in the scaffolds for 2 weeks in vitro, and the activities (proliferation rate and alkaline phosphatase [ALP] activity) of HPDL cells cultured in EDC-SAC and DHT-SAC were compared with those cultured in bovine atelocollagen (BAC) scaffolds cross-linked by EDC (EDC-BAC) and DHT (DHT-BAC), respectively. The proliferation rate of HPDL cells cultured in EDC-SAC was equivalent to that in EDC-BAC, and the ALP activity in EDC-SAC was found to be significantly higher than that in EDC-BAC. In the cross-linking by DHT, the cell proliferation rate and the ALP activity in DHT-SAC were lower than those in DHT-BAC. DHT seemed to provide insufficient cross-linking, and DHT-SAC was found to be breakable and contractile, resulting in less cell activity. In contrast, there was no difference in the thermal stability, porous structure, and cell proliferation rate between EDC-SAC and EDC-BAC. In addition, the collagen helix of EDC-SAC was found to be partially denatured, and this structure resulted in the enhancement of ALP activity of HPDL cells compared with that using EDC-BAC. In conclusion, our results indicate that EDC-SAC could be used as a scaffold for in vitro culture.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1389-1723
EISSN	1347-4421
関連情報	PMID 16233648
	URI http://www.sbj.or.jp/
	URI http://www.sciencedirect.com/science/journal/13891723
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/S1389-1723(04)70224-8