Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film

Yamamoto, Sadaaki; Tanaka, Masaru; Sunami, Hiroshi; Arai, Keiko; Takayama, Aiko; Yamashita, Shigeko; Morita, Yuka; Shimomura, Masatsugu

Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film

Yamamoto, Sadaaki; Tanaka, Masaru; Sunami, Hiroshi et al.

Surface Science, 2006, 600(18), 3785-3791

Permalink : https://hdl.handle.net/2115/15841

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アクセス権	open access
URI	https://hdl.handle.net/2115/15841
タイトル	Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film
著者	Yamamoto, Sadaaki ; Tanaka, Masaru ; Sunami, Hiroshi ; Arai, Keiko ; Takayama, Aiko ; Yamashita, Shigeko ; Morita, Yuka ; Shimomura, Masatsugu
著者名 Yamamoto, Sadaaki
著者名 Tanaka, Masaru
著者名 Sunami, Hiroshi
著者名 Arai, Keiko
著者名 Takayama, Aiko
著者名 Yamashita, Shigeko
著者名 Morita, Yuka
著者名 Shimomura, Masatsugu
言語	英語
キーワード	Atomic force microscopy
	Scanning electron microscopy
	Cell adhesion
	Adsorption
	Self-assembly
	Surface topography
	Fibronectin
	Tissue engineering
発行日	2006-09-15
出版者	Elsevier B.V.
収録物名	Surface Science
巻(号)	600(18)
ページ	3785-3791
抄録	Substratum surface morphology plays a vital roles in cellular behavior. Here, we characterized adsorption of fibronectin (Fn) as a typical cell adhesion protein onto honeycomb-patterned films made of poly(ε-caprolactone) (PCL) by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). In order to determine how cells adhere to a honeycomb-patterned film, focal adhesion of cardiac myocytes (CMYs) and endothelial cells (ECs) on the films were studied by using fluorescence labeling of vinculin. Fn adsorbs around the pore edges to form ring-shaped structures. CMYs and ECs adhere onto the honeycomb-patterned films at focal contact points localized around pore edges distributed over the entire cellular surface. The focal contact points on the honeycomb-patterned films correspond well with the adsorption sites of Fn. We suggest that the cell response to honeycomb-patterned films is associated with the adsorption pattern of Fn on the film.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0039-6028
EISSN	0039-6028
関連情報	URI http://www.sciencedirect.com/science/journal/00396028
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.susc.2006.01.085