Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.

Sudo, Yuki; Iwamoto, Masayuki; Shimono, Kazumi; Kamo, Naoki

Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.

Sudo, Yuki; Iwamoto, Masayuki; Shimono, Kazumi et al.

Journal of Photochemistry and Photobiology B Biology, 2002, 67(3), 171-176

Permalink : https://hdl.handle.net/2115/16004

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アクセス権	open access
URI	https://hdl.handle.net/2115/16004
タイトル	Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.
著者	Sudo, Yuki ; Iwamoto, Masayuki ; Shimono, Kazumi ; Kamo, Naoki
著者名 Sudo, Yuki
著者名 Iwamoto, Masayuki
著者名 Shimono, Kazumi
著者名 Kamo, Naoki
言語	英語
キーワード	Sensory rhodopsin
	Protein–protein interaction
	Photocycle
	Flash-photolysis
	M-intermediate
	Halobacterium salinarum
発行日	2002-07
出版者	Elsevier Science B.V.
収録物名	Journal of Photochemistry and Photobiology B Biology
巻(号)	67(3)
ページ	171-176
抄録	Pharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II) is a receptor of the negative phototaxis of Natronobacterium pharaonis and forms a complex with its transducer pHtrII in membranes. Flash-photolyis of a D75N mutant did not yield the M-intermediate, but an O-like intermediate is observed in a ms time range. We examined the interaction between the D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of 0.1% n-dodecyl-β-maltoside, and the association accelerated the decay of the O of D75N from 15 to 56 s−1. From the decay time constants under varying ratios of D75N and t-Htr, n, the molar ratio of D75N/t-Htr in the complex, and KD, the dissociation constant, were estimated. The value of n was unity and KD was estimated to 146 nM. This KD value can be considered to be the association between the photo-intermediate and t-Htr, which is deduced by the method of estimation. Previously we (Photochem. Photobiol. 74 (2001) 489) reported a KD of 15 μM for the interaction between the wild-type and t-Htr by means of the change in M-decay rates. Therefore, this value should be the KD value for the interaction between M of the wild-type and t-Htr.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1011-1344
EISSN	1011-1344
関連情報	PMID 12167316
関連情報	URI http://www.sciencedirect.com/science/journal/10111344
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/S1011-1344(02)00322-6