Diphosphorylation of the myosin regulatory light chain enhances the tension acting on stress fibers in fibroblasts.

Mizutani, Takeomi; Haga, Hisashi; Koyama, Yoshikazu; Takahashi, Masayuki; Kawabata, Kazushige

Diphosphorylation of the myosin regulatory light chain enhances the tension acting on stress fibers in fibroblasts.

Mizutani, Takeomi; Haga, Hisashi; Koyama, Yoshikazu et al.

Journal of Cellular Physiology, 2006, 209(3), 726-731

Permalink : https://hdl.handle.net/2115/16009

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アクセス権	open access
URI	https://hdl.handle.net/2115/16009
タイトル	Diphosphorylation of the myosin regulatory light chain enhances the tension acting on stress fibers in fibroblasts.
著者	Mizutani, Takeomi ; Haga, Hisashi ; Koyama, Yoshikazu ; Takahashi, Masayuki ; Kawabata, Kazushige
著者名 Mizutani, Takeomi
e-Rad_Researcher 00292045 著者名 Haga, Hisashi
著者名 Koyama, Yoshikazu
e-Rad_Researcher 50241295 著者名 Takahashi, Masayuki
著者名 Kawabata, Kazushige
言語	英語
発行日	2006-12
出版者	Wiley-Liss, Inc.
収録物名	Journal of Cellular Physiology
巻(号)	209(3)
ページ	726-731
抄録	Regulation of the contractile force is crucial for cell migration, cell proliferation, and maintenance of cell morphology. Phosphorylation of the myosin II regulatory light chain (MRLC) is involved in these processes. To show whether the diphosphorylation of MRLC increases the tension acting on stress fibers, changes in the stiffness of fibroblasts expressing wild-type MRLC and a mutant type, which cannot be diphosphorylated, on treatment with lysophosphatidic acid (LPA) were examined by a mechanical-scanning probe microscope (M-SPM). The LPA treatment increased cellular stiffness in the wild-type MRLC expressing cells, while it had no effect on the mutated cells. Immunostaining showed that LPA stimulation induced the diphosphorylation of MRLC. These results suggest that the diphosphorylation of MRLC enhances the tension acting on stress fibers. J. Cell. Physiol. 209: 726-731, 2006. © 2006 Wiley-Liss, Inc.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Copyright © 2006 Wiley-Liss, Inc., Journal of Cellular Physiology,209(3), Pages 726 - 731
PISSN	0021-9541
EISSN	1097-4652
関連情報	PMID 16924661
関連情報	URI http://www.interscience.wiley.com/
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/jcp.20773