High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

Hisano, H.; Kimoto, Y.; Hayakawa, H.; Takeichi, J.; Domae, T.; Hashimoto, R.; Abe, J.; Asano, S.; Kanazawa, A.; Shimamoto, Y.

High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

Hisano, H.; Kimoto, Y.; Hayakawa, H. et al.

Plant Cell Reports, 2004, 22(12), 910-918

Permalink : https://hdl.handle.net/2115/16870

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アクセス権	open access
URI	https://hdl.handle.net/2115/16870
タイトル	High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima
著者	Hisano, H. ; Kimoto, Y. ; Hayakawa, H. ; Takeichi, J. ; Domae, T. ; Hashimoto, R. ; Abe, J. ; Asano, S. ; Kanazawa, A. ; Shimamoto, Y.
著者名 Hisano, H.
著者名 Kimoto, Y.
著者名 Hayakawa, H.
著者名 Takeichi, J.
著者名 Domae, T.
著者名 Hashimoto, R.
e-Rad_Researcher 00192998 著者名 Abe, J.
e-Rad_Researcher 60222585 著者名 Asano, S.
e-Rad_Researcher 30281794 著者名 Kanazawa, A.
著者名 Shimamoto, Y.
言語	英語
キーワード	Agrobacterium tumefaciens
	Beta vulgaris
	Regeneration
	Shoot-base tissue
	Transformation
発行日	2004-07
出版者	Springer Berlin / Heidelberg
収録物名	Plant Cell Reports
巻(号)	22(12)
ページ	910-918
抄録	Communicated by H. Uchimiya Abstract We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and -glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0721-7714
EISSN	1432-203X
関連情報	PMID 15042407
関連情報	URI http://www.springerlink.com
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00299-004-0773-3