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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/16937
タイトル	Isolation and characteristics of carboxypeptidase B from the pyloric ceca of the starfish Asterias amurensis
著者	e-Rad_Researcher 50204855 著者名 Kishimura, Hideki
	著者名 Hayashi, Kenji
言語	英語
キーワード	Asterias amurensis
	Carboxypeptidase B
	Isolation
	Marine invertebrate
	Metalloenzyme
	N-terminal amino acid sequence
	Pyloric cecum
	Starfish
発行日	2002-10
出版者	Elsevier Science Inc.
収録物名	Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
巻(号)	133(2)
ページ	183-189
抄録	Carboxypeptidase B was purified from the pyloric ceca of the starfish Asterias amurensis. The final enzyme preparation was nearly homogeneous in polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 34 000. The optimum pH and temperature of the enzyme for hydrolysis of benzoyl-glycyl--arginine were at approximately pH 7.5 and 55 °C, respectively. The enzyme was unstable at above 50 °C and at below pH 5.0. The enzyme was activated by Co2+, but was inhibited by EDTA and Hg2+. The N-terminal amino acid sequence of A. amurensis carboxypeptidase B was ASFDYNVYHSYQEIMNWITN.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1096-4959
関連情報	URI http://www.sciencedirect.com/science/journal/10964959
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/S1096-4959(02)00130-6

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