Characterization of phospholipase A2 from the pyloric ceca of two species of starfish, Coscinasterias acutispina and Plazaster borealis

Kishimura, Hideki; Hayashi, Kenji

Food Chemistry, 2005, 92(3), 407-411

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アクセス権
open access
URI
https://hdl.handle.net/2115/16942
タイトル
Characterization of phospholipase A2 from the pyloric ceca of two species of starfish, Coscinasterias acutispina and Plazaster borealis
著者
e-Rad_Researcher
50204855
著者名
Kishimura, Hideki
著者名
Hayashi, Kenji
言語
英語
キーワード
Phospholipase A2
Coscinasterias acutispina
Plazaster borealis
Starfish
Marine invertebrate
Pyloric cecum
Polar group specificity
発行日
2005-09
出版者
Elsevier Ltd.
収録物名
Food Chemistry
巻(号)
92(3)
ページ
407-411
抄録
Phospholipase A (PLA) activities in the pyloric ceca and viscera from seven species of marine invertebrates (four starfish, one sea urchin, and two shellfish) were determined. Relatively high PLA specific activities were found in the pyloric ceca of two species of starfish (Coscinasterias acutispina and Plazaster borealis). Phospholipase A2s (PLA2s) were partially purified from the pyloric ceca of the starfish, C. acutispina PLA2 (C-PLA2) and P. borealis PLA2 (P-PLA2). The C-PLA2 and P-PLA2 mainly released oleic acid from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. Temperature optima of the C-PLA2 and P-PLA2 were at around 60 °C and 50 °C, respectively, and pH optima of the C-PLA2 and P-PLA2 were both at around pH 10.0. The activities of the C-PLA2 and P-PLA2 were enhanced by sodium deoxycholate and 1 mM or higher concentration of Ca2+. The C-PLA2 and P-PLA2 did not show the fatty acid specificity for hydrolysis of phosphatidylcholine. Unlike porcine pancreatic PLA2, the C-PLA2 and P-PLA2 hydrolyzed phosphatidylcholine more effectively than phosphatidylethanolamine.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0308-8146
関連情報
URI
http://www.sciencedirect.com/science/journal/03088146
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/j.foodchem.2004.07.031