Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera

Kishimura, Hideki; Hayashi, Kenji

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology, 2002, 132(2), 485-490

Permalink : https://hdl.handle.net/2115/16943

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URI	https://hdl.handle.net/2115/16943
タイトル	Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera
著者	e-Rad_Researcher 50204855 著者名 Kishimura, Hideki
著者	著者名 Hayashi, Kenji
言語	英語
キーワード	Asterina pectinifera
	Calcium ion
	Isolation
	Marine invertebrate
	pH stability
	Pyloric cecum
	Starfish
	Trypsin
発行日	2002-06
出版者	Elsevier Science Inc.
収録物名	Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
巻(号)	132(2)
ページ	485-490
抄録	Trypsin was purified from pyloric ceca of the starfish Asterina Pectinifera by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography. Final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 28 000. Optimum pH and temperature of A. pectinifera trypsin for hydrolysis of Nα-p-Tosyl--arginine methyl ester hydrochloride were approximately pH 8.0 and 55 °C, respectively. A. pectinifera trypsin was unstable at above 50 °C and below pH 5.0, and was not activated by adding Ca2+. The N-terminal amino acid sequence of A. pectinifera trypsin, IVGGHEF, was found.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1096-4959
関連情報	URI http://www.sciencedirect.com/science/journal/10964959
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/S1096-4959(02)00062-3