Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)

Kishimura, Hideki; Saeki, Hiroki; Hayashi, Kenji

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology, 2001, 130(1), 117-123

Permalink : https://hdl.handle.net/2115/16944

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アクセス権
open access
URI
https://hdl.handle.net/2115/16944
タイトル
Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)
著者
Kishimura, Hideki ; Saeki, Hiroki ; Hayashi, Kenji
e-Rad_Researcher
50204855
著者名
Kishimura, Hideki
e-Rad_Researcher
90250505
著者名
Saeki, Hiroki
著者名
Hayashi, Kenji
言語
英語
キーワード
Hepatopancreas
Acid- and heat-stability
Amino acid composition
Inhibitory specificity
Isolation
Marine invertebrate
Squid
Todarodes pacificus
Trypsin inhibitor
発行日
2001-08
出版者
Elsevier Science Inc.
収録物名
Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
巻(号)
130(1)
ページ
117-123
抄録
Trypsin inhibitor was purified from the hepatopancreas of squid (Todarodes pacificus). The final inhibitor preparation was nearly homogeneous by SDS-PAGE with an estimated molecular weight of approximately 6300. The squid trypsin inhibitor was acid- and heat-stable, and active against trypsins from the pyloric ceca of starfish (Asterias amurensis) and saury (Cololabis saira) and porcine pancreatic trypsin. Amino acid composition of the squid trypsin inhibitor was compared with other invertebrate trypsin inhibitors. The squid trypsin inhibitor inhibited the autolysis of walleye pollock (Theragra chalcogramma) myofibrillar proteins.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
1096-4959
関連情報
URI
http://www.sciencedirect.com/science/journal/10964959
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/S1096-4959(01)00415-8