Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.

Takahashi, Eriko; Inanami, Osamu; Ohta, Toshio; Matsuda, Akira; Kuwabara, Mikinori

Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.

Takahashi, Eriko; Inanami, Osamu; Ohta, Toshio et al.

Leukemia Research, 2006, 30(12), 1555-1561

Permalink : https://hdl.handle.net/2115/16953

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アクセス権	open access
URI	https://hdl.handle.net/2115/16953
タイトル	Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.
著者	Takahashi, Eriko ; Inanami, Osamu ; Ohta, Toshio ; Matsuda, Akira ; Kuwabara, Mikinori
著者名 Takahashi, Eriko
e-Rad_Researcher 10193559 著者名 Inanami, Osamu
e-Rad_Researcher 20176895 著者名 Ohta, Toshio
e-Rad_Researcher 90157313 著者名 Matsuda, Akira
著者名 Kuwabara, Mikinori
言語	英語
キーワード	Apoptosis
	Leukemia
	2-Chloro-2'-deoxyadenosine
	Lipid rafts
	Calcium
	L-type Ca2+ channel
発行日	2006-12
出版者	Elsevier Ltd.
収録物名	Leukemia Research
巻(号)	30(12)
ページ	1555-1561
抄録	To clarify the role of lipid rafts in 2-chloro-2′-deoxyadenosine (2CdA; Cladribine)-induced apoptosis, the effects of disruption of lipid rafts by methyl-β-cyclodextrin (MβCD) and filipin on 2CdA-induced apoptosis were investigated in four human acute lymphoblastic leukemia (ALL) cell lines comprised of T cells (MOLT-4, Jurkat) and B cells (NALM, BALL-1). The disruption of lipid rafts significantly inhibited 2CdA-induced apoptosis, indicating the crucial role of lipid rafts in the induction of apoptosis in leukemia cells. These reagents significantly inhibited 2CdA-induced elevation of the intracellular calcium concentration ([Ca2+]i) in MOLT-4 cells, and 2CdA-induced apoptosis was partly inhibited by the Ca2+ chelators BAPTA-AM and EGTA, and the L-type Ca2+ channel blocker nifedipine. On the other hand, they had no effects on the cellular uptake of 2CdA. These results indicated that lipid rafts partly contributed to 2CdA-induced apoptosis by regulating Ca2+ influx via the plasma membrane.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0145-2126
EISSN	0145-2126
関連情報	PMID 16730061
関連情報	URI http://www.sciencedirect.com/science/journal/01452126
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.leukres.2006.04.008