Expression of Bovine Lactoferrin C-lobe in Rhodococcus erythropolis and Its Purification and Characterization

Kim, Woan-Sub; Shimazaki, Kei-ichi; Tamura, Tomohiro

Bioscience Biotechnology and Biochemistry, 2006, 70(11), 2641-2645

Permalink : https://hdl.handle.net/2115/17011

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アクセス権
open access
URI
https://hdl.handle.net/2115/17011
タイトル
Expression of Bovine Lactoferrin C-lobe in Rhodococcus erythropolis and Its Purification and Characterization
著者
Kim, Woan-Sub ; Shimazaki, Kei-ichi ; Tamura, Tomohiro
著者名
Kim, Woan-Sub
著者名
Shimazaki, Kei-ichi
e-Rad_Researcher
30357488
著者名
Tamura, Tomohiro
言語
英語
キーワード
lactoferrin
Rhodococcus
gene expression
antibacterial activity
milk
発行日
2006-11
出版者
Japan Society for Bioscience, Biotechnology, and Agrochemistry
収録物名
Bioscience Biotechnology and Biochemistry
巻(号)
70(11)
ページ
2641-2645
抄録
A Rhodococcus erythropolis expression system for the bovine lactoferrin C-lobe was constructed. The DNA fragments encoding the BLF C-lobe were amplified and cloned into vector pTip LCH1.2. R. erythropolis carrying the pTip-C-lobe was cultured at 30 °C with shaking, and expression of the rBLF C-lobe was induced by adding 1 μg/ml (final concentration) thiostrepton. The rBLF C-lobe was isolated in native and denatured (8 M urea) form by Ni-NTA affinity chromatography. To obtain a bioactive rBLF C-lobe, the protein isolated in the denatured form was refolded by stepwise dialysis against refolding buffers. The antibacterial activity of the rBLF C-lobe was tested by the filter-disc plate assay method. The refolded rBLF C-lobe demonstrated antibacterial activity against selected strains of Escherichia coli.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0916-8451
EISSN
1347-6947
関連情報
PMID
17090917
URI
http://www.jstage.jst.go.jp/
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1271/bbb.60245