Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells

Hoshino, Yoichiro; Scholten, Stefan; von Wiegen, Petra; Lörz, Horst; Kranz, Erhard

Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells

Hoshino, Yoichiro; Scholten, Stefan; von Wiegen, Petra et al.

Sexual Plant Reproduction, 2004, 17(2), 89-95

Permalink : https://hdl.handle.net/2115/18874

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アクセス権	open access
URI	https://hdl.handle.net/2115/18874
タイトル	Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells
著者	Hoshino, Yoichiro ; Scholten, Stefan ; von Wiegen, Petra ; Lörz, Horst ; Kranz, Erhard
e-Rad_Researcher 50301875 著者名 Hoshino, Yoichiro
著者名 Scholten, Stefan
著者名 von Wiegen, Petra
著者名 Lörz, Horst
著者名 Kranz, Erhard
言語	英語
キーワード	Anti-α-tubulin antibody
	Maize
	Microtubule
	Zea mays
	Zygote development
発行日	2004-07
出版者	Springer
収録物名	Sexual Plant Reproduction
巻(号)	17(2)
ページ	89-95
抄録	By using single cell micromanipulation techniques, we developed an immunocytochemical procedure to examine subcellular protein localization in isolated and cultured cells. Localization of microtubules was examined in isolated single egg cells and developing zygotes of maize with anti--tubulin antibodies. In egg cells, a few cortical microtubules were detected but well organized microtubules were rarely observed. In contrast, distinct cortical microtubules and strands of cytoplasmic microtubules radiating from the nucleus to the cell periphery were observed in developing zygotes. Solely cortical microtubules were observed in zygotes up to 7 h after in vitro fertilization. After this time, radiating microtubules additionally appeared, and persisted during zygote development. These results indicate early and pronounced fertilization-induced changes in microtubular organization in the fertilized egg cell of maize.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0934-0882
EISSN	1432-2145
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00497-004-0221-0