HOXD3-overexpression increases integrin alphavbeta3 expression and deprives E-cadherin while it enhances cell motility in A549 cells.

Ohta, Hironori; Hamada, Jun-ichi; Tada, Mitsuhiro; Aoyama, Tetsuya; Furuuchi, Keiji; Takahashi, Yoko; Totsuka, Yasunori; Moriuchi, Tetsuya

HOXD3-overexpression increases integrin alphavbeta3 expression and deprives E-cadherin while it enhances cell motility in A549 cells.

Ohta, Hironori; Hamada, Jun-ichi; Tada, Mitsuhiro et al.

Clinical & Experimental Metastasis, 2006, 23(7-8), 381-390

Permalink : https://hdl.handle.net/2115/18952

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アクセス権	open access
URI	https://hdl.handle.net/2115/18952
タイトル	HOXD3-overexpression increases integrin alphavbeta3 expression and deprives E-cadherin while it enhances cell motility in A549 cells.
著者	Ohta, Hironori ; Hamada, Jun-ichi ; Tada, Mitsuhiro ; Aoyama, Tetsuya ; Furuuchi, Keiji ; Takahashi, Yoko ; Totsuka, Yasunori ; Moriuchi, Tetsuya
著者名 Ohta, Hironori
著者名 Hamada, Jun-ichi
著者名 Tada, Mitsuhiro
著者名 Aoyama, Tetsuya
著者名 Furuuchi, Keiji
著者名 Takahashi, Yoko
著者名 Totsuka, Yasunori
著者名 Moriuchi, Tetsuya
言語	英語
キーワード	Haptotaxis
	E-cadherin
	HOXD3
	Integrin αvβ3
	Migration
	Phagokinesis
	Wound-healing assay
発行日	2006-12
出版者	Springer
収録物名	Clinical & Experimental Metastasis
巻(号)	23(7-8)
ページ	381-390
抄録	We have previously shown that transduction of HOXD3, one of homeobox genes, into human lung cancer A549 cells enhances cell motility, invasion and metastasis. In the present study, we examined the roles of integrin β3 which was up-regulated by HOXD3-overexpression in the HOXD3-induced motility of A549 cells. We first established integrin β3-transfectants and compared their motile activity to those of the HOXD3-transfected, control-transfected and parental cells by three different assays. The integrin β3-transfectants as well as the HOXD3-transfectants formed heterodimer with integrin αv subunit, and showed highly motile activities assessed by haptotaxis or phagokinetic track assay compared to the control transfectants or parental cells. In vitro wound-healing assay revealed that migratory activities were graded as the HOXD3-transfectants > the integrin β3-transfectants > the control transfectants or parental cells. E-cadherin was expressed in the integrin β3-transfectants but not expressed in the HOXD3-transfectants. An addition of function-blocking antibody to E-cadherin into the wound-healing assay promoted the migratory activity of the integrin β3-transfectants, suggesting that E-cadherin prevented the cells from dissociating from the wound edges. These results indicate that increased expression of integrin αv β3 and loss of E-cadherin by HOXD3-overexpression are responsible for the enhanced motility and dissociation.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0262-0898
EISSN	1573-7276
関連情報	PMID 17187229
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10585-006-9047-5