Occurrence of Conjugated Linolenic Acids in Purified Soybean Oil

Kinami, Tomohisa; Horii, Naoto; Narayan, Bhaskar; Arato, Shingo; Hosokawa, Masashi; Miyashita, Kazuo; Negishi, Hironori; Ikuina, Junichi; Noda, Ryuji; Shirasawa, Seiichi

Occurrence of Conjugated Linolenic Acids in Purified Soybean Oil

Kinami, Tomohisa; Horii, Naoto; Narayan, Bhaskar et al.

Journal of the American Oil Chemists Society, 2007, 84(1), 23-29

Permalink : https://hdl.handle.net/2115/20149

このアイテムのアクセス数:1,673件（2026-05-20 01:32 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
JAOC84-1	pdf	181 KB	3,321

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/20149
タイトル	Occurrence of Conjugated Linolenic Acids in Purified Soybean Oil
著者	Kinami, Tomohisa ; Horii, Naoto ; Narayan, Bhaskar ; Arato, Shingo ; Hosokawa, Masashi ; Miyashita, Kazuo ; Negishi, Hironori ; Ikuina, Junichi ; Noda, Ryuji ; Shirasawa, Seiichi
著者名 Kinami, Tomohisa
著者名 Horii, Naoto
著者名 Narayan, Bhaskar
著者名 Arato, Shingo
e-Rad_Researcher 10241374 著者名 Hosokawa, Masashi
e-Rad_Researcher 10182015 著者名 Miyashita, Kazuo
著者名 Negishi, Hironori
著者名 Ikuina, Junichi
著者名 Noda, Ryuji
著者名 Shirasawa, Seiichi
言語	英語
キーワード	CLA
	CLN
	HPLC
	Lipid oxidation
	Soybean oil
発行日	2007-01
出版者	Springer
収録物名	Journal of the American Oil Chemists Society
巻(号)	84(1)
ページ	23-29
抄録	A high-performance liquid chromatographic (HPLC) method is described for the determination of conjugated linoleic acids (CLA) and conjugated linolenic acids (CLN). Methyl esters prepared from purified lipid fractions of soybean oil were analyzed using an HPLC system equipped with photodiode-array detector to detect peaks having maximum absorption around 233 and 275 nm. These peaks were concentrated by AgNO3-silicic acid column chromatography and reversed-phase HPLC. The structural analysis, of dimethyloxazoline (DMOX) derivatized methyl esters, using gas chromatography–mass spectrometry (GC–MS) showed the occurrence of 9,11- and 10,12-CLA and 8,10,13-, 8,10,12-, and 9,11,13-CLN. The comparison of these conjugated fatty acids with authentic isomers by HPLC revealed the presence of isomeric mixtures of CLA [cis (c),trans(t) or t,c and t,t] and CLN (c,t,t or t,t,c and t,t,t). Traces of 9,11- and 10,12-CLA (c,t or t,c) were found in crude oil. CLN isomers (8,10,12-18:3 and 9,11,13-18:3) were found to be forming during the bleaching phase of soybean oil processing. 8,10,13-CLN and 9,11- and 10,12-CLA (t,t) were only found in soybean oil after the deodorization step. CLN contents in commercial soybean oil varied from 387 to 1,316 mg/kg oil. A decreased level of bleaching earth and temperature resulted in a reduced CLN content. It is possible that CLN would be derived from the linoleate hydroperoxides formed during the processing and storage of soybean oil.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0003-021X
EISSN	1558-9331
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11746-006-1005-5