Silencing of Exogenous DNA in Cultured Cells

Ochiai, Hiroshi; Harashima, Hideyoshi; Kamiya, Hiroyuki

Biological & Pharmaceutical Bulletin, 2006, 29(6), 1294-1296

Permalink : https://hdl.handle.net/2115/20469

このアイテムのアクセス数:1,268件（2026-05-30 23:38 集計）

ファイル	フォーマット	サイズ	閲覧回数	説明
29-6-1294	pdf	182 KB	1,347

アクセス権	open access
URI	https://hdl.handle.net/2115/20469
タイトル	Silencing of Exogenous DNA in Cultured Cells
著者	Ochiai, Hiroshi ; Harashima, Hideyoshi ; Kamiya, Hiroyuki
著者名 Ochiai, Hiroshi
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
著者名 Kamiya, Hiroyuki
言語	英語
キーワード	exogenous DNA
	silencing
	intranuclear disposition
発行日	2006-06
出版者	Pharmaceutical Society of Japan
収録物名	Biological & Pharmaceutical Bulletin
巻(号)	29(6)
ページ	1294-1296
抄録	The intranuclear disposition of exogenous DNA is highly important for the therapeutic effects of the administrated DNA. Naked luciferase-plasmid DNA was transfected into cultured cells including HeLa by electroporation, and the amounts of intranuclear plasmid DNA and luciferase were quantitated at various time points. Decrease in expression efficiency from one copy of the exogenous DNA over time occurred as the case of mouse liver, and its degrees varied between cell lines. These results suggest that exogenous DNA is 'silenced' in the cultured cells as well as in mouse hepatocytes.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0918-6158
EISSN	1347-5215
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1248/bpb.29.1294