Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme

Hori, Mika; Asanuma, Taketoshi; Inanami, Osamu; Kuwabara, Mikinori; Harashima, Hideyoshi; Kamiya, Hiroyuki

Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme

Hori, Mika; Asanuma, Taketoshi; Inanami, Osamu et al.

Biological & Pharmaceutical Bulletin, 2006, 29(6), 1087-1091

Permalink : https://hdl.handle.net/2115/20471

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アクセス権	open access
URI	https://hdl.handle.net/2115/20471
タイトル	Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme
著者	Hori, Mika ; Asanuma, Taketoshi ; Inanami, Osamu ; Kuwabara, Mikinori ; Harashima, Hideyoshi ; Kamiya, Hiroyuki
著者名 Hori, Mika
著者名 Asanuma, Taketoshi
e-Rad_Researcher 10193559 著者名 Inanami, Osamu
著者名 Kuwabara, Mikinori
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
著者名 Kamiya, Hiroyuki
言語	英語
キーワード	Orf17
	MutT
	8-hydroxy-dGTP
	nucleotide pool sanitization
	antisense RNA
発行日	2006-06
出版者	Pharmaceutical Society of Japan
収録物名	Biological & Pharmaceutical Bulletin
巻(号)	29(6)
ページ	1087-1091
抄録	The Escherichia coli Orf17 (NtpA, NudB) protein, a MutT-type enzyme, hydrolyzes oxidized deoxyribonucleotides, including 8-hydroxy-2'-deoxyadenosine 5'-triphosphate and 8-hydroxy-2'-deoxyguanosine 5'-triphosphate, in vitro. To examine its in vivo role(s) in bacteria, plasmid DNAs containing the orf17 gene in the sense and antisense orientations were introduced. When the Orf17 protein was overexpressed in mutT cells, the rpoB mutant frequency was decreased. On the other hand, similar effects were not observed when Orf17 was overexpressed in wild type and orf135 cells. Expression of the antisense RNA of the orf17 gene did not produce an obvious phenotype, such as increased mutant frequency and resistance to ionizing radiation. These results suggest that the role of the Orf17 protein is to back up the MutT function, and to assist in the elimination of 8-hydroxy-2'-deoxyguanosine nucleotides.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0918-6158
EISSN	1347-5215
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1248/bpb.29.1087