Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells

Satou, Kazuya; Kawai, Kazuaki; Kasai, Hiroshi; Harashima, Hideyoshi; Kamiya, Hiroyuki

Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells

Satou, Kazuya; Kawai, Kazuaki; Kasai, Hiroshi et al.

Free Radical Biology and Medicine, 2007, 42(10), 1552-1560

Permalink : https://hdl.handle.net/2115/20538

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アクセス権	open access
URI	https://hdl.handle.net/2115/20538
タイトル	Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells
著者	Satou, Kazuya ; Kawai, Kazuaki ; Kasai, Hiroshi ; Harashima, Hideyoshi ; Kamiya, Hiroyuki
著者名 Satou, Kazuya
著者名 Kawai, Kazuaki
著者名 Kasai, Hiroshi
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
著者名 Kamiya, Hiroyuki
言語	英語
キーワード	Reactive oxygen species
	8-Hydroxy-dGTP
	Mutation
	Mammalian cells
発行日	2007-05-15
出版者	Elsevier
収録物名	Free Radical Biology and Medicine
巻(号)	42(10)
ページ	1552-1560
抄録	The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells. 8-OH-dGTP and supF shuttle plasmid DNA were cointroduced by means of cationic liposomes, and the DNAs replicated in the cells were recovered and then transfected into Escherichia coli. 8-OH-dGTP induced A:T->C:G substitution mutations in the COS-7 cells. This result agrees with previous observations indicating that DNA polymerases misincorporate 8-OH-dGTP opposite A in vitro, and that the oxidized deoxyribonucleotide induces A:T->C:G transversions in E. coli. These results constitute the first direct evidence to show that 8-OH-dGTP actually induces mutations in living mammalian cells.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0891-5849
関連情報	PMID 17448902
関連情報	URI http://www.sciencedirect.com/science/journal/08915849
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.freeradbiomed.2007.02.024