STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells.

Ikeda, Osamu; Sekine, Yuichi; Kakisaka, Michinori; Tsuji, Satoshi; Muromoto, Ryuta; Ohbayashi, Norihiko; Oritani, Kenji; Yoshimura, Akihiko; Matsuda, Tadashi

STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells.

Ikeda, Osamu; Sekine, Yuichi; Kakisaka, Michinori et al.

Biochemical and Biophysical Research Communications, 2007, 358(3), 931-937

Permalink : https://hdl.handle.net/2115/22099

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アクセス権	open access
URI	https://hdl.handle.net/2115/22099
タイトル	STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells.
著者	Ikeda, Osamu ; Sekine, Yuichi ; Kakisaka, Michinori ; Tsuji, Satoshi ; Muromoto, Ryuta ; Ohbayashi, Norihiko ; Oritani, Kenji ; Yoshimura, Akihiko ; Matsuda, Tadashi
著者名 Ikeda, Osamu
著者名 Sekine, Yuichi
著者名 Kakisaka, Michinori
著者名 Tsuji, Satoshi
著者名 Muromoto, Ryuta
著者名 Ohbayashi, Norihiko
著者名 Oritani, Kenji
著者名 Yoshimura, Akihiko
e-Rad_Researcher 20212219 著者名 Matsuda, Tadashi
言語	英語
キーワード	Macrophage
	c-Fms
	Tyrosine phosphorylation
	Adaptor protein
	Migration
	Signal transduction
発行日	2007-07-06
出版者	Elsevier Inc.
収録物名	Biochemical and Biophysical Research Communications
巻(号)	358(3)
ページ	931-937
抄録	Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. Our previous studies also revealed that STAP-2 binds to MyD88 and IKK-α/β, and modulates NF-κB signaling in macrophages. In the present study, we examined physiological roles of the interaction between STAP-2 and c-Fms in Raw 264.7 macrophage cells. Our immunoprecipitation has revealed that c-Fms directly interacts with the PH domain of STAP-2 independently on M-CSF-stimulation. Ectopic expression of STAP-2 markedly suppressed M-CSF-induced tyrosine phosphorylation of c-Fms as well as activation of Akt and extracellular signal regulated kinase. In addition, Raw 264.7 cells over-expressing STAP-2 showed impaired migration in response to M-CSF and wound-healing process. Taken together, our findings demonstrate that STAP-2 directly binds to c-Fms and interferes with the PI3K signaling, which leads to macrophage motility, in Raw 264.7 cells.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0006-291X
EISSN	1090-2104
関連情報	PMID 17512498
関連情報	URI http://www.sciencedirect.com/science/journal/0006291X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2007.05.030