Establishment of a newly improved detection system for NF-κB activity

Matsuda, Mayuko; Tsukiyama, Tadasuke; Bohgaki, Miyuki; Nonomura, Katsuya; Hatakeyama, Shigetsugu

Establishment of a newly improved detection system for NF-κB activity

Matsuda, Mayuko; Tsukiyama, Tadasuke; Bohgaki, Miyuki et al.

Immunology Letters, 2007, 109(2), 175-181

Permalink : https://hdl.handle.net/2115/24260

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アクセス権	open access
URI	https://hdl.handle.net/2115/24260
タイトル	Establishment of a newly improved detection system for NF-κB activity
著者	Matsuda, Mayuko ; Tsukiyama, Tadasuke ; Bohgaki, Miyuki ; Nonomura, Katsuya ; Hatakeyama, Shigetsugu
著者名 Matsuda, Mayuko
著者名 Tsukiyama, Tadasuke
著者名 Bohgaki, Miyuki
著者名 Nonomura, Katsuya
e-Rad_Researcher 70294973 著者名 Hatakeyama, Shigetsugu
言語	英語
キーワード	NF-κB
	Transcription
	Reporter system
	Luciferase
	EGFP
発行日	2007-04-15
出版者	Elsevier B.V.
収録物名	Immunology Letters
巻(号)	109(2)
ページ	175-181
抄録	The transcription factor nuclear factor-κB (NF-κB) plays roles in apoptosis, inflammation and oncogenesis. It is important for biological and medical research to understand when proteins of interest are activated in cells, leading to the establishment of a luciferase/EGFP assay to monitor the activation of transcription factors. Here, we describe an improved reporter system for NF-κB, the NF-κB-activated transgene (NAT) system that can detect NF-κB signalling with high sensitivity and specificity. The NAT system consists of large copy numbers of NF-κB consensus sequence and a minimal promoter derived from the mouse interleukin-2 (IL-2) gene. Furthermore, we generated NAT systems with stable or unstable luciferase/EGFP proteins. Stable and unstable types of luciferase/EGFP are suitable for analyzing the accumulation of and the real-time activity of NF-κB signal, respectively. Our findings suggest that the NAT system is effective for in vivo imaging of NF-κB signalling using cells or animals.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0165-2478
関連情報	PMID 17368808
関連情報	URI http://www.sciencedirect.com/science/journal/01652478
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.imlet.2007.02.007