Enhanced replication of human T-cell leukemia virus type 1 in T cells from transgenic rats expressing human CRM1 that is regulated in a natural manner

Takayanagi, Ryo; Ohashi, Takashi; Yamashita, Eizaburo et al.

Journal of Virology, 2007, 81(11), 5908-5918

Permalink : https://hdl.handle.net/2115/27990

このアイテムのアクセス数:1,450件（2026-05-15 07:41 集計）

閲覧可能ファイル 

ファイル フォーマット サイズ 閲覧回数 説明
JV81-11 pdf 2.16 MB 1,245

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権
open access
URI
https://hdl.handle.net/2115/27990
タイトル
Enhanced replication of human T-cell leukemia virus type 1 in T cells from transgenic rats expressing human CRM1 that is regulated in a natural manner
著者
Takayanagi, Ryo ; Ohashi, Takashi ; Yamashita, Eizaburo ; Kurosaki, Yohei ; Tanaka, Kumiko ; Hakata, Yoshiyuki ; Komoda, Yasumasa ; Ikeda, Satoru ; Tsunetsugu-Yokota, Yasuko ; Tanaka, Yuetsu ; Shida, Hisatoshi
著者名
Takayanagi, Ryo
著者名
Ohashi, Takashi
著者名
Yamashita, Eizaburo
著者名
Kurosaki, Yohei
著者名
Tanaka, Kumiko
著者名
Hakata, Yoshiyuki
著者名
Komoda, Yasumasa
著者名
Ikeda, Satoru
著者名
Tsunetsugu-Yokota, Yasuko
著者名
Tanaka, Yuetsu
e-Rad_Researcher
00144395
著者名
Shida, Hisatoshi
言語
英語
発行日
2007-06
出版者
American Society for Microbiology
収録物名
Journal of Virology
巻(号)
81(11)
ページ
5908-5918
抄録
Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia (ATL). To develop a better animal model for the investigation of HTLV-1 infection, we established a transgenic (Tg) rat carrying the human CRM1 (hCRM1) gene, which encodes a viral RNA transporter that is a species-specific restriction factor. At first we found that CRM1 expression is elaborately regulated through a pathway involving protein kinase C during lymphocyte activation, initially by posttranscriptional and subsequently by transcriptional mechanisms. This fact led us to use an hCRM1-containing bacterial artificial chromosome clone, which would harbor the entire regulatory and coding regions of the CRM1 gene. The Tg rats expressed hCRM1 protein in a manner similar to expression of intrinsic rat CRM1 in various organs. HTLV-1-infected T-cell lines derived from these Tg rats produced 100- to 10,000-fold more HTLV-1 than did T cells from wild-type rats, and the absolute levels of HTLV-1 were similar to those produced by human T cells. We also observed enhancement of the dissemination of HTLV-1 to the thymus in the Tg rats after intraperitoneal inoculation, although the proviral loads were low in both wild-type and Tg rats. These results support the essential role of hCRM1 in proper HTLV-1 replication and suggest the importance of this Tg rat as an animal model for HTLV-1.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
Copyright © 2007 American Society for Microbiology
PISSN
0022-538X
関連情報
PMID
17360758
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1128/JVI.02811-06