OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2

Maekawa, Satoshi; Mori, Daisuke; Nishiya, Tadashi; Takikawa, Osamu; Horinouchi, Takahiro; Nishimoto, Arata; Kajita, Emi; Miwa, Soichi

OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2

Maekawa, Satoshi; Mori, Daisuke; Nishiya, Tadashi et al.

Biochimica et Biophysica Acta (BBA) : Molecular Cell Research, 2007, 1773(6), 1000-1006

Permalink : https://hdl.handle.net/2115/28237

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アクセス権	open access
URI	https://hdl.handle.net/2115/28237
タイトル	OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2
著者	Maekawa, Satoshi ; Mori, Daisuke ; Nishiya, Tadashi ; Takikawa, Osamu ; Horinouchi, Takahiro ; Nishimoto, Arata ; Kajita, Emi ; Miwa, Soichi
著者名 Maekawa, Satoshi
著者名 Mori, Daisuke
著者名 Nishiya, Tadashi
著者名 Takikawa, Osamu
著者名 Horinouchi, Takahiro
e-Rad_Researcher 90396325 著者名 Nishimoto, Arata
著者名 Kajita, Emi
e-Rad_Researcher 40157706 著者名 Miwa, Soichi
言語	英語
キーワード	OCTN2
	OCTN2VT
	Splicing
	Carnitine
	N-glycosylation
	Endoplasmic reticulum
発行日	2007-06
出版者	Elsevier B.V.
収録物名	Biochimica et Biophysica Acta (BBA) : Molecular Cell Research
巻(号)	1773(6)
ページ	1000-1006
抄録	A novel organic cation transporter OCTN2 is indispensable for carnitine transport across plasma membrane and subsequent fatty acid metabolism in the mitochondria. Here, we report a novel splice variant of OCTN2 (OCTN2VT), in which a 72-base-pair sequence located in the first intron of OCTN2 gene was spliced between exons 1 and 2 of OCTN2, causing the insertion of 24 amino acids in the first extracellular loop of OCTN2. Despite the similarity between OCTN2 and OCTN2VT regarding primary structure and tissue distribution, their biochemical characteristics were significantly different. OCTN2 was expressed on the plasma membrane with robust N-glycosylation, whereas OCTN2VT was retained in the endoplasmic reticulum (ER) with poor N-glycosylation. In addition, the retention in the ER caused no carnitine uptake into the cells. These results demonstrate that the biochemical and functional characteristics of OCTN2VT are distinct from OCTN2 due to the insertion of 24 amino acids in the first extracellular loop.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0167-4889
関連情報	PMID 17509700
関連情報	URI http://www.sciencedirect.com/science/journal/01674889
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbamcr.2007.04.005