Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus.

Ito, Shigeaki; Hamada, Shigeki; Yamaguchi, Kozo et al.

Biochemical and Biophysical Research Communications, 2007, 360(3), 640-645

Permalink : https://hdl.handle.net/2115/28242

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アクセス権
open access
URI
https://hdl.handle.net/2115/28242
タイトル
Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus.
著者
Ito, Shigeaki ; Hamada, Shigeki ; Yamaguchi, Kozo ; Umene, Shingo ; Ito, Hiroyuki ; Matsui, Hirokazu ; Ozawa, Tadahiro ; Taguchi, Hidenori ; Watanabe, Jun ; Wasaki, Jun ; Ito, Susumu
著者名
Ito, Shigeaki
著者名
Hamada, Shigeki
著者名
Yamaguchi, Kozo
著者名
Umene, Shingo
著者名
Ito, Hiroyuki
e-Rad_Researcher
90109504
著者名
Matsui, Hirokazu
著者名
Ozawa, Tadahiro
著者名
Taguchi, Hidenori
著者名
Watanabe, Jun
著者名
Wasaki, Jun
著者名
Ito, Susumu
言語
英語
キーワード
Cellobiose 2-epimerase
Ruminococcus albus
Rumen
Cloning
Sequencing
発行日
2007-08-31
出版者
Elsevier Inc.
収録物名
Biochemical and Biophysical Research Communications
巻(号)
360(3)
ページ
640-645
抄録
Cellobiose 2-epimerase (EC 5.1.3.11) was first identified in 1967 as an extracellular enzyme that catalyzes the reversible epimerization between cellobiose and 4-O-β-d-glucopyranosyl-d-mannose in a culture broth of Ruminococcus albus 7 (ATCC 27210T). Here, for the first time, we describe the purification of cellobiose 2-epimerase from R. albus NE1. The enzyme was found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose. The gene encoding cellobiose 2-epimerase comprises 1170 bp (389 amino acids) and is present as a single copy in the genome. The deduced amino acid sequence of the mature enzyme contains the possible catalytic residues Arg52, His243, Glu246, and His374. Sequence analysis shows the gene shares a very low level of homology with N-acetyl-d-glucosamine 2-epimerases (EC 5.1.3.8), but no significant homology to any other epimerases reported to date.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0006-291X
EISSN
1090-2104
関連情報
PMID
17612504
URI
http://www.sciencedirect.com/science/journal/0006291X
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/j.bbrc.2007.06.091