open access

https://hdl.handle.net/2115/29924

Changes in stiffness of yeast cells during cell cycle by passability through micromachined channel arrays

英語

saccharomyces cerevisiae

1998-03-26

SPIE-The International Society for Optical Engineering

Proceedings of Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications

3258

188-194

Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, 26-27 January 1998, San Jose, California

Possibility of cell sorting by cellular deformability was examined using yeast cells and previously described microchannel arrays. Cells harvested at every hour during incubation were washed and suspended in sorbitol solution at a concentration of O.D. 0.3. An aliquot of each suspension was caused to flow through the microchannel concentration of O.D. 0.3. An aliquot of each suspension was caused to flow through the microchannel arrays by applying 20 cmH2O suction. Cells at two hours of incubation could not enter into the microchannels, while cells at 4-5 hours of incubation could enter into the microchannels despite their larger size due to budding than the preceding ones and some few cells were observed to pass through 8 micrometers width microchannels. The number of cells that could enter into the microchannels decreased at 7-8 hours and re-increased at 9- 10 hours, but the synchronism in this second cycle appeared to decrease. Protoplasts prepared by treatment with zymolyase from cells at 4-5 hours of incubation showed no appreciable resistance to the microchannel passage.

会議発表論文

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Copyright 1998 Society of Photo-Optical Instrumentation Engineers. This paper was published in Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, 3258, 188-194, and is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

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