Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase

Saburi, Wataru; Hondoh, Hironori; Unno, Hideaki; Okuyama, Masayuki; Mori, Haruhide; Nakada, Toshitaka; Matsuura, Yoshiki; Kimura, Atsuo

Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase

Saburi, Wataru; Hondoh, Hironori; Unno, Hideaki et al.

Acta Crystallographica Section F : Structural Biology and Crystallization Communications, 2007, 63(9), 774-776

Permalink : https://hdl.handle.net/2115/30136

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アクセス権	open access
URI	https://hdl.handle.net/2115/30136
タイトル	Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase
著者	Saburi, Wataru ; Hondoh, Hironori ; Unno, Hideaki ; Okuyama, Masayuki ; Mori, Haruhide ; Nakada, Toshitaka ; Matsuura, Yoshiki ; Kimura, Atsuo
e-Rad_Researcher 00598089 著者名 Saburi, Wataru
著者名 Hondoh, Hironori
著者名 Unno, Hideaki
e-Rad_Researcher 00344490 著者名 Okuyama, Masayuki
e-Rad_Researcher 80241363 著者名 Mori, Haruhide
著者名 Nakada, Toshitaka
著者名 Matsuura, Yoshiki
e-Rad_Researcher 90186312 著者名 Kimura, Atsuo
言語	英語
キーワード	dextran glucosidase
	Streptococcus mutans
	α-amylase family
発行日	2007-09
出版者	International Union of Crystallography
収録物名	Acta Crystallographica Section F : Structural Biology and Crystallization Communications
巻(号)	63(9)
ページ	774-776
抄録	Dextran glucosidase from Streptococcus mutans is an exo-hydrolase that acts on the nonreducing terminal α-1,6-glucosidic linkage of oligosaccharides and dextran with a high degree of transglucosylation. Based on amino-acid sequence similarity, this enzyme is classified into glycoside hydrolase family 13. Recombinant dextran glucosidase was purified and crystallized by the hanging-drop vapour-diffusion technique using polyethylene glycol 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 72.72, b = 86.47, c = 104.30 Å. A native data set was collected to 2.2 Å resolution from a single crystal.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1744-3091
EISSN	1744-3091
関連情報	PMID 17768352
関連情報	URI http://journals.iucr.org/
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1107/S174430910703936X