Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration

Azuma, Chinatsu; Tohyama, Harukazu; Nakamura, Hideki; Kanaya, Fuminori; Yasuda, Kazunori

Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration

Azuma, Chinatsu; Tohyama, Harukazu; Nakamura, Hideki et al.

Journal of Biomechanics, 2007, 40(10), 2184-2190

Permalink : https://hdl.handle.net/2115/30163

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アクセス権	open access
URI	https://hdl.handle.net/2115/30163
タイトル	Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration
著者	Azuma, Chinatsu ; Tohyama, Harukazu ; Nakamura, Hideki ; Kanaya, Fuminori ; Yasuda, Kazunori
著者名 Azuma, Chinatsu
e-Rad_Researcher 60301884 著者名 Tohyama, Harukazu
著者名 Nakamura, Hideki
著者名 Kanaya, Fuminori
e-Rad_Researcher 20166507 著者名 Yasuda, Kazunori
言語	英語
キーワード	Collagen
	Fibroblast
	Patellar tender
	TGF-β
	Tissue engineering
発行日	2007
出版者	Elsevier Ltd.
収録物名	Journal of Biomechanics
巻(号)	40(10)
ページ	2184-2190
抄録	A tissue-cultured tendon matrix infiltrated with cultured fibroblasts can be regarded as an ideal tissue-engineered tendon model. To clarify the role of TGF-β in a tissue-cultured tendon matrix during ex vivo cellular infiltration, the present ex vivo study was conducted to test the following hypothesis that antibody neutralization of TGF-β enhances weakening of the collagen fascicles of the patellar tendon matrix in response to ex vivo fibroblast infiltration. In skeletally mature female rabbits, fibroblasts were isolated from the right patellar tendons using an explant culture technique, and the left patellar tendons underwent multiple freeze/thaw treatment with liquid nitrogen to obtain an acellular tendon matrix. Each acellular tendon was placed in a collagen gel containing cultured fibroblasts and then incubated with or without anti-TGF-β1 antibody for 6 weeks. We found that antibody neutralization of TGF-β enhanced the decrease in the tensile strength and tensile modulus of the collagen fascicles of the patellar tendon matrix in response to ex vivo fibroblast infiltration. The present study indicates a possibility that TGF-β may have a role in suppressing the material deterioration of the fascicles in the tendon during ex vivo cellular infiltration.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0021-9290
関連情報	PMID 17462658
関連情報	URI http://www.sciencedirect.com/science/journal/00219290
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jbiomech.2006.10.023